Cdk2 activity is associated with depolarization of mitochondrial membrane potential during apoptosis
- Title
- Cdk2 activity is associated with depolarization of mitochondrial membrane potential during apoptosis
- Author
- 임형신
- Keywords
- Apoptosis; Caspase cascade; Cdk2; Cytochrome c release; Mitochondrial membrane depolarization
- Issue Date
- 2003-06
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v. 305, issue. 4, page. 974-980
- Abstract
- In this study we show that panaxadiol, a ginseng saponin with a dammarane skeleton, induces apoptotic cell death by depolarization of mitochondrial membrane potential in human hepatoma SK-HEP-1 cells. Sequential activation of caspases-9, -3, and -7, but not of caspase-8, occurs after mitochondrial membrane depolarization and cytochrome c release from the mitochondria of panaxadiol-treated cells. Moreover, Cdk2 kinase activity, but not Cdc2 kinase activity, is markedly upregulated in the early stages of apoptosis. Olomoucine or roscovitine, specific Cdks inhibitors, effectively prevent mitochondrial membrane depolarization as well as apoptotic cell death in panaxadiol-treated cells. Thus, panaxadiol-treatment induces cell death-dependent activation of Cdk2 kinase activity, which is functionally associated with depolarization of mitochondrial membrane potential and subsequent cytochrome c release.
- URI
- https://www.sciencedirect.com/science/article/pii/S0006291X03008702?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/155933
- ISSN
- 0006-291X
- DOI
- 10.1016/S0006-291X(03)00870-2
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML