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Thermally triggered self-assembly of kappa-casein amyloid nanofibrils and their nanomechanical properties

Title
Thermally triggered self-assembly of kappa-casein amyloid nanofibrils and their nanomechanical properties
Author
염봉준
Keywords
Amyloids; Self-assembly; Nanomechanical property
Issue Date
2019-09
Publisher
ELSEVIER SCI LTD
Citation
POLYMER, v. 179, article no. UNSP 121626
Abstract
Understanding the self-assembly process of amyloid nanofibrils is important in protein engineering for their potential uses in material sciences and medical applications. Here, we investigated the nanomechanical properties and structures of kappa-casein nanofibrils during thermally induced amyloidogenesis. After treatment with dithiothreitol to reduce disulfide bonds, kappa-casein from bovine milk was incubated at various temperatures from 37 to 95 degrees C. Twisted mature nanofibrils were only obtained at high thermal energies of 95 degrees C with fast growth kinetics. Nanomechanical studies using atomic force microscopy show the highest Young's moduli (2.6 +/- 0.4 GPa) for mature fibrils when compared to those of oligomers and protofibrils (0.6 +/- 0.1 and 2.2 +/- 0.3 GPa, respectively). These enhanced mechanical properties are mainly attributed to the twisted cores of the mature fibrils containing beta-sheet stacks. Amyloidogenesis of kappa-casein is highly dependent on the magnitude of thermal energy, determining both the types and shapes of the nanofibrils and their different mechanical properties.
URI
https://www.sciencedirect.com/science/article/pii/S003238611930610X?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/153757
ISSN
0032-3861; 1873-2291
DOI
10.1016/j.polymer.2019.121626
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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