Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 염봉준 | - |
dc.date.accessioned | 2020-09-11T01:11:45Z | - |
dc.date.available | 2020-09-11T01:11:45Z | - |
dc.date.issued | 2019-09 | - |
dc.identifier.citation | POLYMER, v. 179, article no. UNSP 121626 | en_US |
dc.identifier.issn | 0032-3861 | - |
dc.identifier.issn | 1873-2291 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S003238611930610X?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/153757 | - |
dc.description.abstract | Understanding the self-assembly process of amyloid nanofibrils is important in protein engineering for their potential uses in material sciences and medical applications. Here, we investigated the nanomechanical properties and structures of kappa-casein nanofibrils during thermally induced amyloidogenesis. After treatment with dithiothreitol to reduce disulfide bonds, kappa-casein from bovine milk was incubated at various temperatures from 37 to 95 degrees C. Twisted mature nanofibrils were only obtained at high thermal energies of 95 degrees C with fast growth kinetics. Nanomechanical studies using atomic force microscopy show the highest Young's moduli (2.6 +/- 0.4 GPa) for mature fibrils when compared to those of oligomers and protofibrils (0.6 +/- 0.1 and 2.2 +/- 0.3 GPa, respectively). These enhanced mechanical properties are mainly attributed to the twisted cores of the mature fibrils containing beta-sheet stacks. Amyloidogenesis of kappa-casein is highly dependent on the magnitude of thermal energy, determining both the types and shapes of the nanofibrils and their different mechanical properties. | en_US |
dc.description.sponsorship | KC and JL acknowledge the financial support from the National Research Foundation of Korea through the Korea Ministry of Science, ICT & Future Planning (MSIP), The National Creative Research Initiative Program for "Intelligent Hybrids Research Center" (No. 2010-0018290). This work was also supported by the research fund of Hanyang University (HY-2018). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Amyloids | en_US |
dc.subject | Self-assembly | en_US |
dc.subject | Nanomechanical property | en_US |
dc.title | Thermally triggered self-assembly of kappa-casein amyloid nanofibrils and their nanomechanical properties | en_US |
dc.type | Article | en_US |
dc.relation.volume | 179 | - |
dc.identifier.doi | 10.1016/j.polymer.2019.121626 | - |
dc.relation.page | 121626-121626 | - |
dc.relation.journal | POLYMER | - |
dc.contributor.googleauthor | Lee, Jubong | - |
dc.contributor.googleauthor | Lee, Ji-Hye | - |
dc.contributor.googleauthor | Palk, Seung R. | - |
dc.contributor.googleauthor | Yeom, Bongjun | - |
dc.contributor.googleauthor | Char, Kookheon | - |
dc.relation.code | 2019003729 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF CHEMICAL ENGINEERING | - |
dc.identifier.pid | byeom | - |
dc.identifier.orcid | https://orcid.org/0000-0001-8914-0947 | - |
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