MOF-Bacteriophage Biosensor for Highly Sensitive and Specific Detection of Staphylococcus aureus
- Title
- MOF-Bacteriophage Biosensor for Highly Sensitive and Specific Detection of Staphylococcus aureus
- Author
- 김기현
- Keywords
- Staphylococcus aureus; bacteriophages; MOF; fluorescence; detection
- Issue Date
- 2017-09
- Publisher
- AMER CHEMICAL SOC
- Citation
- ACS APPLIED MATERIALS & INTERFACES, v. 9, no. 39, page. 33589-33598
- Abstract
- To produce a sensitive and specific biosensor for Staphylococcus aureus, bacteriophages have been interfaced with a water-dispersible and environmentally stable metal-organic framework (MOF), NH2-MIL-53(Fe). The conjugation of the MOF with bacteriophages has been achieved through the use of glutaraldehyde as cross-linker. Highly sensitive detection of S. aureus in both synthetic and real samples was realized by the proposed MOF-bacteriophage' biosensor based on the photoluminescence quenching phenomena; limit of detection (31 CFU/mL) and range of detection (40 to 4 X 10(8) CFU/mL). This is the first report exploiting the use of an MOF-bacteriophage complex for the biosensing of S. aureus. The results of our study highlight that-the proposed biosensor is more sensitive than most of the previous methods while exhibiting some advanced features like specificity) regenerability, extended range of linear 'detection, and stability for long-term storage (even at room temperature).
- URI
- https://pubs.acs.org/doi/10.1021/acsami.7b07818https://repository.hanyang.ac.kr/handle/20.500.11754/115708
- ISSN
- 1944-8244
- DOI
- 10.1021/acsami.7b07818
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > CIVIL AND ENVIRONMENTAL ENGINEERING(건설환경공학과) > Articles
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