Plasma-polymerized antifouling biochips for label-free measurement of protease activity in cell culture media
- Title
- Plasma-polymerized antifouling biochips for label-free measurement of protease activity in cell culture media
- Author
- 김영필
- Keywords
- Protease activity; Plasma copolymerization; Antifouling; Nonspecific adsorption; Surface plasmon resonance
- Issue Date
- 2019-02
- Publisher
- ELSEVIER SCIENCE SA
- Citation
- SENSORS AND ACTUATORS B-CHEMICAL, v. 281, Page. 527-534
- Abstract
- We report polyethylene glycol (PEG)-grafting antifouling surfaces using a plasma copolymerized (PcP) technique to monitor protease activity in complex media. By varying the mixing ratio of the PEG and ethylenediamine (EDA) precursors, the PcP-PEG-EDA (PcP-PE) film was able to easily control surface amine density with good preservation of the internal PEG structure. We found that nonspecific protein adsorption was dramatically reduced in serum-containing media on the PcP-PE films, as opposed to that on plasma polymerized-EDA (PP-E) films without PEG. When SPR sensor chips coated with PcP-PE film were employed to detect protease activity, biotinylated luciferase probes (luciferase-peptide-biotin) on streptavidin-conjugated SPR chips enabled real-time and label-free measurement of matrix metalloproteinase activity in cell culture media. Owing to its excellent antifouling ability, this newly developed method boasts minimal nonspecific binding and can serve as a biochip platform to promote a wide range of applications in the biological field.
- URI
- https://www.sciencedirect.com/science/article/pii/S0925400518319002?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/103681
- ISSN
- 0925-4005
- DOI
- 10.1016/j.snb.2018.10.123
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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