Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김영필 | - |
dc.date.accessioned | 2019-05-09T06:45:25Z | - |
dc.date.available | 2019-05-09T06:45:25Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | SENSORS AND ACTUATORS B-CHEMICAL, v. 281, Page. 527-534 | en_US |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0925400518319002?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/103681 | - |
dc.description.abstract | We report polyethylene glycol (PEG)-grafting antifouling surfaces using a plasma copolymerized (PcP) technique to monitor protease activity in complex media. By varying the mixing ratio of the PEG and ethylenediamine (EDA) precursors, the PcP-PEG-EDA (PcP-PE) film was able to easily control surface amine density with good preservation of the internal PEG structure. We found that nonspecific protein adsorption was dramatically reduced in serum-containing media on the PcP-PE films, as opposed to that on plasma polymerized-EDA (PP-E) films without PEG. When SPR sensor chips coated with PcP-PE film were employed to detect protease activity, biotinylated luciferase probes (luciferase-peptide-biotin) on streptavidin-conjugated SPR chips enabled real-time and label-free measurement of matrix metalloproteinase activity in cell culture media. Owing to its excellent antifouling ability, this newly developed method boasts minimal nonspecific binding and can serve as a biochip platform to promote a wide range of applications in the biological field. | en_US |
dc.description.sponsorship | The work was supported by the Development of Platform Technology for Innovative Medical Measurements Program (KRISS-2017-GP2017-0020) from the Korea Research Institute of Standards and Science, the Nano Material Technology Development Programs (NRF-2014M3A7B6020163, NRF-2017M3A7B4041754, and NRF-2018M3D1A1058814), the Bio & Medical Technology Development Program (NRF-2015M3A9D7029894), and Research Program (NRF-2016R1A2B2011744) of the National Research Foundation (NRF) funded by the Ministry of Science and ICT and Development of Nanotechnology-based Quantitative Surface Mass Spectrometric Platform Technique to Detect Low-mass Biomolecules funded by the MOTIE. This work was also supported by Basic Science Research Program (No. 2012R1A6A1029029) through the NRF funded by the Ministry of Education. In addition, support by Dr. Alexei Nefedov (KIT) from the CRG running the HE-SGM beamline and XPS/NEXAFS end station and staff at HZB/BESSY II is gratefully acknowledged. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE SA | en_US |
dc.subject | Protease activity | en_US |
dc.subject | Plasma copolymerization | en_US |
dc.subject | Antifouling | en_US |
dc.subject | Nonspecific adsorption | en_US |
dc.subject | Surface plasmon resonance | en_US |
dc.title | Plasma-polymerized antifouling biochips for label-free measurement of protease activity in cell culture media | en_US |
dc.type | Article | en_US |
dc.relation.volume | 281 | - |
dc.identifier.doi | 10.1016/j.snb.2018.10.123 | - |
dc.relation.page | 527-534 | - |
dc.relation.journal | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.contributor.googleauthor | Park, Jisoo | - |
dc.contributor.googleauthor | Kim, Gae Baik | - |
dc.contributor.googleauthor | Lippitz, Andreas | - |
dc.contributor.googleauthor | Kim, Young Mi | - |
dc.contributor.googleauthor | Jung, Donggeun | - |
dc.contributor.googleauthor | Unger, Wolfgang E. S. | - |
dc.contributor.googleauthor | Kim, Young-Pil | - |
dc.contributor.googleauthor | Lee, Tae Geol | - |
dc.relation.code | 2019002468 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | ypilkim | - |
dc.identifier.researcherID | A-5437-2009 | - |
dc.identifier.orcid | http://orcid.org/0000-0001-7234-1320 | - |
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