An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses
- Title
- An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses
- Author
- 진언선
- Keywords
- Chaetoceros neogracile; immunogold labeling; ice-binding site
- Issue Date
- 2014-12
- Publisher
- Federation AMER SOC EXP BIOL
- Citation
- FASEB journa, 2014, 28(11), P.4924-4935
- Abstract
- The structure and function of the Antarctic marine diatom Chaetoceros neogracile antifreeze protein (Cn-AFP), as well as its expression levels and characteristics of the ice-binding site, were analyzed in the present study. In silico analysis revealed that the Cn-AFP promoter contains both light- and temperature-responsive elements. Northern and Western blot analyses demonstrated that both Cn-AFP transcript and protein expression were strongly and rapidly stimulated by freezing, as well as temperature and high light stress. Immunogold labeling revealed that Cn-AFP is preferentially localized to the intracellular space near the chloroplast membrane. Recombinant Cn-AFP had clear antifreeze activity. Protein-folding simulation was used to predict the putative ice-binding sites in Cn-AFP, and site-directed mutagenesis of the Cn-AFP b-face confirmed their identification.Gwak, Y., Jung, W., Lee, Y., Kim, J. S., Kim, C. G., Ju, J.-H., Song, C., Hyun, J.-K., Jin, E. An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses.
- URI
- https://www.fasebj.org/doi/10.1096/fj.14-256388
- ISSN
- 0892-6638; 1530-6860
- DOI
- 10.1096/fj.14-256388
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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