Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 진언선 | - |
dc.date.accessioned | 2018-03-20T05:26:05Z | - |
dc.date.available | 2018-03-20T05:26:05Z | - |
dc.date.issued | 2014-12 | - |
dc.identifier.citation | FASEB journa, 2014, 28(11), P.4924-4935 | en_US |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.issn | 1530-6860 | - |
dc.identifier.uri | https://www.fasebj.org/doi/10.1096/fj.14-256388 | - |
dc.description.abstract | The structure and function of the Antarctic marine diatom Chaetoceros neogracile antifreeze protein (Cn-AFP), as well as its expression levels and characteristics of the ice-binding site, were analyzed in the present study. In silico analysis revealed that the Cn-AFP promoter contains both light- and temperature-responsive elements. Northern and Western blot analyses demonstrated that both Cn-AFP transcript and protein expression were strongly and rapidly stimulated by freezing, as well as temperature and high light stress. Immunogold labeling revealed that Cn-AFP is preferentially localized to the intracellular space near the chloroplast membrane. Recombinant Cn-AFP had clear antifreeze activity. Protein-folding simulation was used to predict the putative ice-binding sites in Cn-AFP, and site-directed mutagenesis of the Cn-AFP b-face confirmed their identification.Gwak, Y., Jung, W., Lee, Y., Kim, J. S., Kim, C. G., Ju, J.-H., Song, C., Hyun, J.-K., Jin, E. An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses. | en_US |
dc.description.sponsorship | This work was supported by a Korea Carbo Capture and Sequestration (CCS) Research and Development (R&D) Center (KCRC), grant funded by the Korean government [Ministry of Science, Information, Communications and Technology (ICT), and Future Planning; National Research Foundation (NRF)-2014M1A8A1049273]. This research was also supported by the NRF of Korea (grant NRF-C1ABA001-2010-0020501) | en_US |
dc.language.iso | en | en_US |
dc.publisher | Federation AMER SOC EXP BIOL | en_US |
dc.subject | Chaetoceros neogracile | en_US |
dc.subject | immunogold labeling | en_US |
dc.subject | ice-binding site | en_US |
dc.title | An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses | en_US |
dc.type | Article | en_US |
dc.relation.no | 11 | - |
dc.relation.volume | 28 | - |
dc.identifier.doi | 10.1096/fj.14-256388 | - |
dc.relation.page | 4924-4935 | - |
dc.relation.journal | FASEB JOURNAL | - |
dc.contributor.googleauthor | Gwak, Yunho | - |
dc.contributor.googleauthor | Jung, Woongsic | - |
dc.contributor.googleauthor | Lee, Yew | - |
dc.contributor.googleauthor | Kim, Ji Sook | - |
dc.contributor.googleauthor | Kim, Chul Geun | - |
dc.contributor.googleauthor | Ju, Ji-Hyun | - |
dc.contributor.googleauthor | Song, Chihong | - |
dc.contributor.googleauthor | Hyun, Jae-Kyung | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.relation.code | 2014029566 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | esjin | - |
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