Failure of Tooth Formation Mediated by miR-135a Overexpression via BMP Signaling
- Title
- Failure of Tooth Formation Mediated by miR-135a Overexpression via BMP Signaling
- Author
- 김계성
- Keywords
- microRNA; Bmp receptors; miRNA expression; lentivirus; miRNA inhibitor; tooth development
- Issue Date
- 2014-06
- Publisher
- SAGE PUBLICATIONS INC
- Citation
- JOURNAL OF DENTAL RESEARCH, 2014, 93(6), P.571-575
- Abstract
- MicroRNAs (miRNAs) are known to regulate a variety of gene functions in many tissues and organs, but their expression and function in tooth development are not well-understood. A specific miRNA, miR-135a, was determined to be highly expressed at the bud stage. Interestingly, after the cap stage, miR-135a was expressed in the epithelium and mesenchyme but not in the inner enamel epithelium. To identify the relationship between miR-135a and its putative target genes, Bmpr-Ia and Bmpr-Ib, in early tooth development, miR-135a was ectopically overexpressed with a lentivirus. This overexpression resulted in the repression of Bmpr-Ia and Bmpr-Ib. Furthermore, miR-135a inhibited both Bmpr-Ia and Bmpr-Ib transcription. BMP2 proteins were expressed ectopically in tooth germs during the cap stage to determine the relationship between miR-135a and BMP signaling in early tooth development. When miR-135a was ectopically expressed, no tooth formation was observed after 4 wk of incubation in the kidney capsule. This study suggested that Bmp signaling, specifically Bmpr-Ia and Bmpr-Ib, regulates tooth formation via miR-135a.
- URI
- http://journals.sagepub.com/doi/abs/10.1177/0022034514529303?journalCode=jdrb
- ISSN
- 0022-0345; 1544-0591
- DOI
- 10.1177/0022034514529303
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML