김계성
2018-03-16T02:44:22Z
2018-03-16T02:44:22Z
2014-06
JOURNAL OF DENTAL RESEARCH, 2014, 93(6), P.571-575
0022-0345
1544-0591
http://journals.sagepub.com/doi/abs/10.1177/0022034514529303?journalCode=jdrb
MicroRNAs (miRNAs) are known to regulate a variety of gene functions in many tissues and organs, but their expression and function in tooth development are not well-understood. A specific miRNA, miR-135a, was determined to be highly expressed at the bud stage. Interestingly, after the cap stage, miR-135a was expressed in the epithelium and mesenchyme but not in the inner enamel epithelium. To identify the relationship between miR-135a and its putative target genes, Bmpr-Ia and Bmpr-Ib, in early tooth development, miR-135a was ectopically overexpressed with a lentivirus. This overexpression resulted in the repression of Bmpr-Ia and Bmpr-Ib. Furthermore, miR-135a inhibited both Bmpr-Ia and Bmpr-Ib transcription. BMP2 proteins were expressed ectopically in tooth germs during the cap stage to determine the relationship between miR-135a and BMP signaling in early tooth development. When miR-135a was ectopically expressed, no tooth formation was observed after 4 wk of incubation in the kidney capsule. This study suggested that Bmp signaling, specifically Bmpr-Ia and Bmpr-Ib, regulates tooth formation via miR-135a.
This study was supported by a grant from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A101578). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.
en
SAGE PUBLICATIONS INC
microRNA
Bmp receptors
miRNA expression
lentivirus
miRNA inhibitor
tooth development
Failure of Tooth Formation Mediated by miR-135a Overexpression via BMP Signaling
Article
6
93
10.1177/0022034514529303
571-575
JOURNAL OF DENTAL RESEARCH
Kim, E.J
Lee, M.J.
Li, L.
Yoon, K.S.
Kim, K.S.
Jung, H.S
2014032925
S
COLLEGE OF MEDICINE[S]
DEPARTMENT OF MEDICINE
ks66kim