Intravascular optical imaging of high-risk plaques in vivo by targeting macrophage mannose receptors
- Title
- Intravascular optical imaging of high-risk plaques in vivo by targeting macrophage mannose receptors
- Author
- 유홍기
- Keywords
- HUMAN ATHEROSCLEROTIC PLAQUES; MATRIX METALLOPROTEINASES; CORONARY-DISEASE; NANOPARTICLES; EXPRESSION; CHITOSAN; CELLS; BIODISTRIBUTION; PATHOGENESIS; INFLAMMATION
- Issue Date
- 2016-03
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v. 6, NO 22608, Page. 1-11
- Abstract
- Macrophages mediate atheroma expansion and disruption, and denote high-risk arterial plaques. Therefore, they are substantially gaining importance as a diagnostic imaging target for the detection of rupture-prone plaques. Here, we developed an injectable near-infrared fluorescence (NIRF) probe by chemically conjugating thiolated glycol chitosan with cholesteryl chloroformate, NIRF dye (cyanine 5.5 or 7), and maleimide-polyethylene glycol-mannose as mannose receptor binding ligands to specifically target a subset of macrophages abundant in high-risk plaques. This probe showed high affinity to mannose receptors, low toxicity, and allowed the direct visualization of plaque macrophages in murine carotid atheroma. After the scale-up of the MMR-NIRF probe, the administration of the probe facilitated in vivo intravascular imaging of plaque inflammation in coronary-sized vessels of atheromatous rabbits using a custom-built dual-modal optical coherence tomography (OCT)-NIRF catheter-based imaging system. This novel imaging approach represents a potential imaging strategy enabling the identification of high-risk plaques in vivo and holds promise for future clinical implications.
- URI
- https://www.nature.com/articles/srep22608http://hdl.handle.net/20.500.11754/36039
- ISSN
- 2045-2322
- DOI
- 10.1038/srep22608
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > ELECTRICAL AND BIOMEDICAL ENGINEERING(전기·생체공학부) > Articles
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