Mesoporous platinum nanozyme-based competitive immunoassay for sensitive detection of 25-hydroxyvitamin D
- Title
- Mesoporous platinum nanozyme-based competitive immunoassay for sensitive detection of 25-hydroxyvitamin D
- Author
- 성기훈
- Keywords
- Vitamin D; Nanozymes; Mesoporous nanoparticles; Competitive immunoassay; Colorimetry
- Issue Date
- 2022-09-06
- Publisher
- ELSEVIER
- Citation
- MATERIALS LETTERS
- Abstract
- In this study, mesoporous platinum nanozymes (MPNs) were fabricated by a surfactant-based self-assembly method and then used to develop a novel competitive immunoassay for sensitive detection of 25-hydroxyvitamin D3 (25(OH)VD3). The MPNs showed enhanced peroxidase-like activity that catalyzes the reaction between hydrogen peroxide (H2O2) and 3,3 ',5,5 '-tetramethylbenzidine (TMB). The excellent peroxidase-like MPNs were employed to develop a competitive immunoassay for the sensitive colorimetric detection of 25(OH)VD3. The immunoassay detected 25(OH)VD3 in the range of 1.6 to 100 ng/mL, with a detection limit of 1.0 ng/mL. The recovery values of 25(OH)VD3 from the spiked human serum samples were between 104% and 95%. These results indicate the applicability of MPNs as alternatives for natural enzymes and the feasibility for sensitive 25 (OH)VD3 detection.
- URI
- https://www.sciencedirect.com/science/article/pii/S0167577X2201641Xhttps://repository.hanyang.ac.kr/handle/20.500.11754/189629
- ISSN
- 0167-577X
- DOI
- https://doi.org/10.1016/j.matlet.2022.133286
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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