Separation of Progressive Motile Sperm from Mouse Semen Using On-chip Chemotaxis
- Title
- Separation of Progressive Motile Sperm from Mouse Semen Using On-chip Chemotaxis
- Author
- 황승용
- Keywords
- SYSTEM; CELLS; INJECTION; INFERTILITY; PATTERNS; IN-VITRO FERTILIZATION; MICROFLUIDIC DEVICES
- Issue Date
- 2012-01
- Publisher
- Japan Society for Analytical Chemistry
- Citation
- Analytical Sciences, v. 28, NO. 1, Page. 27-32
- Abstract
- We present a novel method for the separation of progressive motile sperm from non-progressive motile and immotile sperm. This separation was accomplished by inducing chemotaxis along a longitudinal chemical gradient in a microchip composed of a biocompatible polydimethysiloxane layer and a glass substrate. In a preliminary experiment using fluorescent rhodamine B as a marker, we verified that a chemical gradient was generated by diffusion within the microchannel. We used acetylcholine as a chemoattractant to evaluate the chemotactic response of sperm. We tested the response to a 1/2 to 1/64 dilution series of acetylcholine. The results of a mouse sperm chemotaxis assay showed that progressive motile sperm swam predominantly toward the outlet at an optimal chemical gradient of 0.625 (mg/ml)/mm of acetylcholine. This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection.
- URI
- https://www.jstage.jst.go.jp/article/analsci/28/1/28_1_27/_articlehttps://repository.hanyang.ac.kr/handle/20.500.11754/181631
- ISSN
- 0910-6340;1348-2246
- DOI
- 10.2116/analsci.28.27
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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