Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2023-05-31T01:04:16Z | - |
dc.date.available | 2023-05-31T01:04:16Z | - |
dc.date.issued | 2012-01 | - |
dc.identifier.citation | Analytical Sciences, v. 28, NO. 1, Page. 27-32 | - |
dc.identifier.issn | 0910-6340;1348-2246 | - |
dc.identifier.uri | https://www.jstage.jst.go.jp/article/analsci/28/1/28_1_27/_article | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/181631 | - |
dc.description.abstract | We present a novel method for the separation of progressive motile sperm from non-progressive motile and immotile sperm. This separation was accomplished by inducing chemotaxis along a longitudinal chemical gradient in a microchip composed of a biocompatible polydimethysiloxane layer and a glass substrate. In a preliminary experiment using fluorescent rhodamine B as a marker, we verified that a chemical gradient was generated by diffusion within the microchannel. We used acetylcholine as a chemoattractant to evaluate the chemotactic response of sperm. We tested the response to a 1/2 to 1/64 dilution series of acetylcholine. The results of a mouse sperm chemotaxis assay showed that progressive motile sperm swam predominantly toward the outlet at an optimal chemical gradient of 0.625 (mg/ml)/mm of acetylcholine. This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection. | - |
dc.description.sponsorship | a Korea Research Foundation grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-311-D00326). | - |
dc.language | en | - |
dc.publisher | Japan Society for Analytical Chemistry | - |
dc.subject | SYSTEM | - |
dc.subject | CELLS | - |
dc.subject | INJECTION | - |
dc.subject | INFERTILITY | - |
dc.subject | PATTERNS | - |
dc.subject | IN-VITRO FERTILIZATION | - |
dc.subject | MICROFLUIDIC DEVICES | - |
dc.title | Separation of Progressive Motile Sperm from Mouse Semen Using On-chip Chemotaxis | - |
dc.type | Article | - |
dc.relation.no | 1 | - |
dc.relation.volume | 28 | - |
dc.identifier.doi | 10.2116/analsci.28.27 | - |
dc.relation.page | 27-32 | - |
dc.relation.journal | Analytical Sciences | - |
dc.contributor.googleauthor | Ko, Yong-Jun | - |
dc.contributor.googleauthor | Maeng, Joon-Ho | - |
dc.contributor.googleauthor | Lee, Byung-Chul | - |
dc.contributor.googleauthor | Lee, Seungwoo | - |
dc.contributor.googleauthor | Hwang, Seung Yong | - |
dc.contributor.googleauthor | Ahn, Yoomin | - |
dc.sector.campus | E | - |
dc.sector.daehak | 과학기술융합대학 | - |
dc.sector.department | 의약생명과학과 | - |
dc.identifier.pid | syhwang | - |
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