Microfluidic Biochips for Simple Impedimetric Detection of Thrombin Based on Label-free DNA Aptamers
- Title
- Microfluidic Biochips for Simple Impedimetric Detection of Thrombin Based on Label-free DNA Aptamers
- Author
- 황승용
- Keywords
- DNA Aptamer; Microfluidics; Thrombin; Electrochemical impedance spectroscopy; Anti-thrombin aptamer
- Issue Date
- 2017-06
- Publisher
- 한국바이오칩학회
- Citation
- BioChip Journal, v. 11, NO. 2, Page. 109-115
- Abstract
- This paper presents a proof-of-concept microfluidic aptamer-based sensor for thrombin point of -care testing using electrochemical impedance spectroscopy. The disposable biosensor is composed of a polydimethylsiloxane (PDMS) channel layer over a glass substrate. The substrate surface has an Au working electrode and a Pt reference/counter electrode. In this study, human thrombin was used as a biomarker for disease diagnosis. An unlabeled aptamer specific to thrombin was immobilized on a working electrode and electrochemical impedance was measured as thrombin was injected into the biosensor. Thrombin was detected by measuring change in impedance. The proposed biochip had a detection range of 0.1-100,000 ng mL(-1) for thrombin concentration and a limit of detection of 0.1 ng mL(-1). The results indicated that our biochip could be an effective tool for other molecular diagnostic systems.
- URI
- https://link.springer.com/article/10.1007%2Fs13206-016-1203-7https://repository.hanyang.ac.kr/handle/20.500.11754/181574
- ISSN
- 1976-0280;2092-7843
- DOI
- 10.1007/s13206-016-1203-7
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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