Pretreatment of Corn Stover Using an Extremely Low-Liquid Ammonia (ELLA) Method for the Effective Utilization of Sugars in Simultaneous Saccharification and Fermentation (SSF) of Ethanol
- Title
- Pretreatment of Corn Stover Using an Extremely Low-Liquid Ammonia (ELLA) Method for the Effective Utilization of Sugars in Simultaneous Saccharification and Fermentation (SSF) of Ethanol
- Author
- KIM TAE HYUN
- Keywords
- liquid ammonia; corn stover; alkaline pretreatment; enzyme digestibility; lignocellulosic biomass; simultaneous saccharification and fermentation (SSF)
- Issue Date
- 2021-09
- Publisher
- MDPI
- Citation
- FERMENTATION-BASEL, v. 7, NO. 3, article no. 191, Page. 1-15
- Abstract
- Extremely low-liquid ammonia (ELLA) pretreatment using aqueous ammonia was investigated in order to enhance the enzymatic saccharification of corn stover and subsequent ethanol production. In this study, corn stover was treated with an aqueous ammonia solution at different ammonia loading rates (0.1, 0.2, and 0.3 g NH3/g biomass) and various liquid-to-solid (L/S) ratios (0.55, 1.12, and 2.5). The ELLA pretreatment was conducted at elevated temperatures (90-150 degrees C) for an extended period (24-120 h). Thereafter, the pretreated material was saccharified by enzyme digestion and subjected to simultaneous saccharification and fermentation (SSF) tests. The effects of key parameters on both glucan digestibility and xylan digestibility were analyzed using analysis of variance (ANOVA). Under optimal pretreatment conditions (L/S = 2.5, 0.1 g-NH3/g-biomass, 150 degrees C), 81.2% glucan digestibility and 61.1% xylan digestibility were achieved. The highest ethanol yield achieved on the SSF tests was 85.4%. The ethanol concentration was 14.5 g/L at 96 h (pretreatment conditions: liquid-to-solid ratio (L/S) = 2.5, 0.1 g-NH3/g-biomass, 150 degrees C, 24 h. SSF conditions: microorganism Saccharomyces cerevisiae (D(5)A), 15 FPU/g-glucan, CTech2, 3% w/v glucan, 37 degrees C, 150 rpm).
- URI
- https://www.mdpi.com/2311-5637/7/3/191https://repository.hanyang.ac.kr/handle/20.500.11754/178684
- ISSN
- 2311-5637;2311-5637
- DOI
- 10.3390/fermentation7030191
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
- Files in This Item:
- 81700_KIM TAE HYUN.pdfDownload
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