The regulatory impact of RNA-binding proteins on microRNA targeting
- Title
- The regulatory impact of RNA-binding proteins on microRNA targeting
- Author
- 남진우
- Issue Date
- 2021-08
- Publisher
- NATURE PORTFOLIO
- Citation
- NATURE COMMUNICATIONS, v. 12, NO. 1, article no. 5057, Page. 1-15
- Abstract
- miRNAs are loaded into Argonaute protein and repress complementary mRNA targets. Here the authors show the unappreciated role of RNA binding proteins for efficient miRNA targeting and expand the current understanding of miRNA targeting. Argonaute is the primary mediator of metazoan miRNA targeting (MT). Among the currently identified >1,500 human RNA-binding proteins (RBPs), there are only a handful of RBPs known to enhance MT and several others reported to suppress MT, leaving the global impact of RBPs on MT elusive. In this study, we have systematically analyzed transcriptome-wide binding sites for 150 human RBPs and evaluated the quantitative effect of individual RBPs on MT efficacy. In contrast to previous studies, we show that most RBPs significantly affect MT and that all of those MT-regulating RBPs function as MT enhancers rather than suppressors, by making the local secondary structure of the target site accessible to Argonaute. Our findings illuminate the unappreciated regulatory impact of human RBPs on MT, and as these RBPs may play key roles in the gene regulatory network governed by metazoan miRNAs, MT should be understood in the context of co-regulating RBPs.
- URI
- https://www.nature.com/articles/s41467-021-25078-5https://repository.hanyang.ac.kr/handle/20.500.11754/177885
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-021-25078-5
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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