Reactive Oxygen Species Driven Angiogenesis by Inorganic Nanorods

Abstract

The exact mechanism of angiogenesis by europium hydroxide nanorods was unclear. In this study we have showed that formation of reactive oxygen species (H2O2 and O-2 center dot-) is involved in redox signaling pathways during angiogenesis, important for cardiovascular and ischemic diseases. Here we used single-walled carbon nanotube sensor array to measure the single-molecule efflux of H2O2 and a HPLC method for the determination of O-2 center dot- from endothelial cells in response to proangiogenic factors. Additionally, reactive oxygen species-mediated angiogenesis using inorganic nanorods was observed in transgenic (flila:EGFP) zebrafish embryos.