Single-cell analysis of gastric pre-cancerous and cancer lesions reveals cell lineage diversity and intratumoral heterogeneity
- Title
- Single-cell analysis of gastric pre-cancerous and cancer lesions reveals cell lineage diversity and intratumoral heterogeneity
- Author
- 조수영
- Keywords
- Neoplasms; Tumors; Oncology; Including cancer and carcinogens; RC254-282
- Issue Date
- 2022-01
- Publisher
- NATURE PORTFOLIO
- Citation
- npj Precision Oncology, Vol 6, Iss 1, Pp 1-11 (2022)
- Abstract
- Single-cell transcriptomic profiles analysis has proposed new insights for understanding the behavior of human gastric cancer (GC).
GC offers a unique model of intratumoral heterogeneity. However, the specific classes of cells involved in carcinogenetic passage,
and the tumor microenvironment of stromal cells was poorly understood. We characterized the heterogeneous cell population of
precancerous lesions and gastric cancer at the single-cell resolution by RNA sequencing. We identified 10 gastric cell subtypes and
showed the intestinal and diffuse-type cancer were characterized by different cell population. We found that the intestinal and
diffuse-type cancer cells have the differential metaplastic cell lineages: intestinal-type cancer cells differentiated along the intestinal
metaplasia lineage while diffuse-type cancer cells resemble de novo pathway. We observed an enriched CCND1 mutation in
premalignant disease state and discovered cancer-associated fibroblast cells harboring pro-stemness properties. In particular,
tumor cells could be categorized into previously proposed molecular subtypes and harbored specific subtype of malignant cell with
high expression level of epithelial-myofibroblast transition which was correlated with poor clinical prognosis. In addition to
intratumoral heterogeneity, the analysis revealed different cellular lineages were responsible for potential carcinogenetic pathways.
Single-cell transcriptomes analysis of gastric pre-cancerous lesions and cancer may provide insights for understanding GC cell
behavior, suggesting potential targets for the diagnosis and treatment of GC.
- URI
- https://www.proquest.com/docview/2623204176?accountid=11283https://repository.hanyang.ac.kr/handle/20.500.11754/171073
- ISSN
- 2397-768X
- DOI
- 10.1038/s41698-022-00251-1
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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