Flavin-containing monooxygenase 1-catalysed N,N-dimethylamphetamine N-oxidation
- Title
- Flavin-containing monooxygenase 1-catalysed N,N-dimethylamphetamine N-oxidation
- Author
- 유혜현
- Keywords
- N,N-dimethylamphetamine (DMA); flavin-containing monooxygenase; N-oxidation; N,N-dimethylamphetamine N-oxide (DMANO)
- Issue Date
- 2009-09
- Publisher
- TAYLOR & FRANCIS LTD
- Citation
- XENOBIOTICA, v. 39, no. 9, page. 680-686
- Abstract
- N,N-dimethylamphetamine (DMA) is a methamphetamine analogue known to be a weaker central nervous system stimulant than methamphetamine. Although a major metabolite of DMA is known to be DMA N-oxide (DMANO), which may be catalysed by flavin-containing monooxygenase (FMO), the specific enzyme(s) involved in this biotransformation has not been identified. In this study, the specific enzyme(s) involved with DMA N-oxidation was characterized by several assays. When DMA was incubated with different human recombinant drug-metabolizing enzymes, including FMOs and cytochrome P450s (CYPs), the formation of DMANO by FMO1 was the most predominant. The Michaelis-Menten kinetic constants for DMA N-oxidation by FMO1 were: K(m) of 44.5 mu M, V(max) of 7.59 nmol min(-1) mg(-1) protein, and intrinsic clearance of 171 mu l min(-1) mg(-1) protein, which was about twelve-fold higher than that by FMO3. Imipramine, an FMO1-specific inhibitor, selectively inhibited DMA N oxidation. The resulting data showed that DMA N oxidation is mainly mediated by FMO1.
- URI
- https://eds.a.ebscohost.com/eds/detail/detail?vid=0&sid=0a45de6b-7471-471e-85f6-be6e3ce98c23%40sdc-v-sessmgr02&bdata=Jmxhbmc9a28mc2l0ZT1lZHMtbGl2ZQ%3d%3d#AN=edselc.2-52.0-70350510900&db=edselchttps://repository.hanyang.ac.kr/handle/20.500.11754/165993
- ISSN
- 0049-8254; 1366-5928
- DOI
- 10.1080/00498250902998699
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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