Anti-inflammatory action of mollugin and its synthetic derivatives in HT-29 human colonic epithelial cells is mediated through inhibition of NF-kappa B activation
- Title
- Anti-inflammatory action of mollugin and its synthetic derivatives in HT-29 human colonic epithelial cells is mediated through inhibition of NF-kappa B activation
- Author
- 최한곤
- Keywords
- Mollugin; Chemokine; Intercellular adhesion molecule (ICAM)-1; NF-κB; Intestinal inflammation
- Issue Date
- 2009-11
- Publisher
- ELSEVIER SCIENCE BV
- Citation
- EUROPEAN JOURNAL OF PHARMACOLOGY, v. 622, no. 1-3, page. 52-57
- Abstract
- Mollugin is the active compound of Rubia cordifolia, which has been used as a traditional Chinese medicine for the treatment of various inflammatory diseases including arthritis and uteritis. In the present study, we investigated for the first time the inhibitory effects and the mechanisms of action of mollugin (M1) and its synthetic derivatives (M2–M4) on tumor necrosis factor (TNF)-α-induced inflammatory responses in HT-29 human colon epithelial cells. Treatment with M1 and its derivatives M2–M4 significantly inhibited TNF-α-induced attachment of U937 monocytic cells to HT-29 cells, which mimics the initial phase of colon inflammation. TNF-α-induced mRNA induction of the chemokines, monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8, and the intercellular cell adhesion molecule (ICAM)-1, which are involved in adhesion between leukocytes and epithelial cells, was suppressed by M1–M4, and M1 was the most efficacious. In addition, M1–M4 significantly suppressed TNF-α-induced NF-κB transcriptional activity. Such NF-κB inhibitory activity of M1–M4 (20 µM) correlated with their ability to suppress TNF-α-induced chemokine expression and U937 monocytic cell adhesion to HT-29 colonic epithelial cells. Treatment of HT-29 cells with M1 and PDTC, a NF-κB inhibitor, synergistically suppressed both TNF-α-induced NF-κB activation and monocytic cell adhesion to HT-29 cells. These results suggest that M1–M4 inhibit TNF-α-induced expression of inflammatory molecules via NF-κB, and that M1, a potent NF-κB inhibitor, may be a valuable new drug candidate for the treatment of colon inflammation.
- URI
- https://www.sciencedirect.com/science/article/pii/S0014299909007742https://repository.hanyang.ac.kr/handle/20.500.11754/165960
- ISSN
- 0014-2999
- DOI
- 10.1016/j.ejphar.2009.09.008
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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