Toxoplasma gondii GRA8-derived peptide immunotherapy improves tumor targeting of colorectal cancer
- Title
- Toxoplasma gondii GRA8-derived peptide immunotherapy improves tumor targeting of colorectal cancer
- Author
- 양철수
- Keywords
- Metabolism; Mitochondria; Toxoplasma gondii GRA8 peptide; Tumor-targeting
- Issue Date
- 2020-01
- Publisher
- Impact Journals
- Citation
- Oncotarget, v. 11, issue. 1, Page. 62-73
- Abstract
- Targeted tumor and efficient, specific biological drug delivery in vivo has been one of the main challenges in protein-based cancer-targeted therapies. Mitochondria are potential therapeutic targets for various anti-cancer drugs. We have previously reported that protein kinase Cα-mediated phosphorylation of Toxoplasma gondii GRA8 is required for mitochondrial trafficking and regulating the interaction of the C-terminal of GRA8 with ATP5A1/SIRT3 in mitochondria. Furthermore, SIRT3 facilitates ATP5A1 deacetylation, mitochondrial activation, and subsequent antiseptic activity in vivo. Herein we developed a recombinant acidity-triggered rational membrane (ATRAM)-conjugated multifunctional GRA8 peptide (rATRAM-G8-M/AS) comprising ATRAM as the cancer-targeting cell-penetrating peptide, and essential/ minimal residues for mitochondrial targeting or ATP5A1/SIRT3 binding. This peptide construct showed considerably improved potency about cancer cell death via mitochondria activity and biogenesis compared with rGRA8 alone in HCT116 human carcinoma cells, reaching an IC
- URI
- https://www.scopus.com/record/display.uri?eid=2-s2.0-85078813858&origin=inward&txGid=c5e6645b0d02bdd8d926b45d74f3ede0https://repository.hanyang.ac.kr/handle/20.500.11754/163291
- ISSN
- 1949-2553
- DOI
- 10.18632/oncotarget.27417
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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