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Single-Molecular AFM Probing of Specific DNA Sequencing Using RecA-promoted Homologous Pairing and Strand Exchange

Title
Single-Molecular AFM Probing of Specific DNA Sequencing Using RecA-promoted Homologous Pairing and Strand Exchange
Author
성기훈
Issue Date
2000-03
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v. 72, issue. 6, page. 1288-1293
Abstract
The specific sequence in a linearlized double-stranded DNA target has been identified at a single-molecular level by atomic force microscopy (AFM). This was accomplished using RecA-coated, single-stranded DNA probes which were paired with a specific complementary DNA sequence in a linear double-stranded DNA target by strand-exchange reaction at a homologous sequence site with target DNA. The sites of interaction between the nucleoprotein filaments and the double-stranded DNA targets were directly visualized by AFM in solution containing 4 mM magnesium acetate. Measurements of the position of RecA-coated probes paired to individual target DNA showed that DNA probes specifically paired at their corresponding homologous target sequences. Strand exchange promoted by RecA and the visualization by AFM provided a rapid and efficient way to identify homologous sequence on a single-molecule target DNA.
URI
https://pubs.acs.org/doi/10.1021/ac990893hhttps://repository.hanyang.ac.kr/handle/20.500.11754/161824
ISSN
0003-2700
DOI
10.1021/ac990893h
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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