Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 성기훈 | - |
dc.date.accessioned | 2021-04-28T00:33:42Z | - |
dc.date.available | 2021-04-28T00:33:42Z | - |
dc.date.issued | 2000-03 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v. 72, issue. 6, page. 1288-1293 | en_US |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/ac990893h | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/161824 | - |
dc.description.abstract | The specific sequence in a linearlized double-stranded DNA target has been identified at a single-molecular level by atomic force microscopy (AFM). This was accomplished using RecA-coated, single-stranded DNA probes which were paired with a specific complementary DNA sequence in a linear double-stranded DNA target by strand-exchange reaction at a homologous sequence site with target DNA. The sites of interaction between the nucleoprotein filaments and the double-stranded DNA targets were directly visualized by AFM in solution containing 4 mM magnesium acetate. Measurements of the position of RecA-coated probes paired to individual target DNA showed that DNA probes specifically paired at their corresponding homologous target sequences. Strand exchange promoted by RecA and the visualization by AFM provided a rapid and efficient way to identify homologous sequence on a single-molecule target DNA. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.title | Single-Molecular AFM Probing of Specific DNA Sequencing Using RecA-promoted Homologous Pairing and Strand Exchange | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1021/ac990893h | - |
dc.relation.journal | ANALYTICAL CHEMISTRY | - |
dc.contributor.googleauthor | Niimi, Tomohisa | - |
dc.contributor.googleauthor | Yanagida, Yasuko | - |
dc.contributor.googleauthor | Kobatake, Eiry | - |
dc.contributor.googleauthor | Aizawa, Masuo | - |
dc.contributor.googleauthor | Seong, Gi Hun | - |
dc.relation.code | 2007200599 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF BIONANO ENGINEERING | - |
dc.identifier.pid | ghseong | - |
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