Eotaxin induces migration of PBL-2H3 mast cells via a Rac-ERK-dependent pathway
- Title
- Eotaxin induces migration of PBL-2H3 mast cells via a Rac-ERK-dependent pathway
- Author
- 정일엽
- Keywords
- Eotaxin; Chemotaxis; RBL-2H3; Rac; ERK
- Issue Date
- 2002-09
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Citation
- Biochemical and Biophysical Research Communications, v. 298, issue. 3, page, 392-397
- Abstract
- Eotaxin is a potent chemokine that acts via CC chemokine receptor 3 (CCR3) to induce chemotaxis, mainly on eosinophils. Here we show that eotaxin also induces chemotactic migration in rat basophilic leukemia (RBL-2H3) mast cells. This effect was dose-dependently inhibited by compound X, a selective CCR3 antagonist, indicating that, as in eosinophils, the effect was mediated by CCR3. Eotaxin-induced cell migration was completely blocked in RBL-RacN17 cells expressing a dominant negative Rac1 mutant, suggesting a crucial role for Rac1 in eotaxin signaling to chemotactic migration. ERK activation also proved essential for eotaxin signaling and it too was absent in RBL-RacN17 cells. Finally, we found that activation of Rac and ERK was correlated with eotaxin-induced actin reorganization known to be necessary for cell motility. It thus appears that Rac1 acts upstream of ERK to signal chemotaxis in these cells, and that a Rac-ERK-dependent cascade mediates the eotaxin-induced chemotactic motility of RBL-2H3 mast cells.
- URI
- https://www.sciencedirect.com/science/article/pii/S0006291X02024324https://repository.hanyang.ac.kr/handle/20.500.11754/157422
- ISSN
- 0006-291X; 1090-2104
- DOI
- 10.1016/S0006-291X(02)02432-4
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML