CAAT/enhancer binding protein activates an enhancer in the glutamine synthetase distal 5 '-flanking sequence
- Title
- CAAT/enhancer binding protein activates an enhancer in the glutamine synthetase distal 5 '-flanking sequence
- Author
- 류종석
- Keywords
- glutamine synthetase; gene expression; adipocytes; CAAT/enhancer binding proteins; diabetes mellitus; obesity
- Issue Date
- 2002-01
- Publisher
- ELSEVIER SCIENCE INC
- Citation
- ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, v. 397, no. 2, page. 258-261
- Abstract
- The glutamine synthetase (GS) gene is expressed at high levels in several cell types, including astrocytes, pericentral hepatocytes, and adipocytes. During hormone-mediated adipocyte differentiation of 3T3-L1 cells, GS gene expression increases several hundred fold. We previously reported that elements in the distal 5′-flanking sequence and intron-1 participate in establishing the temporal pattern of GS transcription during adipocyte differentiation. To examine the role of the distal 5′-flanking region in regulating adipocyte-specific GS expression, GS–CAT fusion genes were constructed and analyzed in transiently transfected 3T3-L1 cells. In this way, adipocyte differentiation-responsive enhancer activity was localized to a 422-bp sequence that occurs about 3.5 kb upstream from the transcription start site. This sequence includes several putative C/EBP binding sites and is activated by ectopic expression of C/EBPα in NIH-3T3 cells. Thus, our data indicate that C/EBPα has the capacity to activate functional C/EBP sites in the GS gene distal 5′-flanking region.
- URI
- https://www.sciencedirect.com/science/article/pii/S0003986101926669?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/156906
- ISSN
- 0003-9861
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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