Dextromethorphan alters gene expression in rat brain hippocampus and cortex
- Title
- Dextromethorphan alters gene expression in rat brain hippocampus and cortex
- Author
- 이영식
- Issue Date
- 2003-05
- Publisher
- PROFESSOR D A SPANDIDOS
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, page. 559-568
- Abstract
- Dextromethorphan is a widely used anti-tussive drug with non-competitive antagonistic
effects on excitatory amino acid receptors of the N-methyl-D-aspartate (NMDA) type.
This study examined the effect of daily dextromethorphan administration on gene
expression in rat brain hippocampus and cortex regions using Rat 5K cDNA microarrays.
Triplicate microarray assays were performed at each time point (1, 3 and 10 days),
and results were confirmed using semi-quantitative RT-PCR on a subset of
differentially expressed cDNA. The microarray analysis proved able to detect
changes in gene expression following dextromethorphan injection. Moreover, these
changes were mostly mediated by an NMDA receptor. The hippocampus region showed
more alterations in gene expression than cerebral cortex following dextromethorphan
treatment. The expression of many glutamate-induced apoptosis-related genes, and
NO-dependent apoptosis-associated genes, was down-regulated. Expression of
anti-apoptotic genes, such as nucleophosmin/B23, Rab2, MAP kinase kinase and CREB
binding protein, was up-regulated by dextromethorphan. Angiogenesis is likely to be
inhibited in our system due to observed down-regulation of VEGF-associated genes.
Expression of some SNARE genes was up-regulated in rat brain hippocampus and cortex
regions after dextromethorphan injection
- URI
- https://www.spandidos-publications.com/10.3892/ijmm.11.5.559https://repository.hanyang.ac.kr/handle/20.500.11754/155774
- ISSN
- 1107-3756
- DOI
- 10.3892/ijmm.11.5.559
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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