Construction of recombinant BCGs overexpressing antigen 85 complex and their protective efficacy against Mycobacterium tuberculosis infection in a mouse model
- Title
- Construction of recombinant BCGs overexpressing antigen 85 complex and their protective efficacy against Mycobacterium tuberculosis infection in a mouse model
- Author
- 김효준
- Keywords
- Tuberculosis Vaccine; Mycobacteria Bovi; Mycobacteria Tuberculosis Antigen
- Issue Date
- 2004-08
- Publisher
- 대한결핵 및 호흡기학회
- Citation
- Tuberculosis and Respiratory Diseases, v. 57, No, 2, Page. 125-131
- Abstract
- Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed overexpression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN- γ production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.
- URI
- https://www.scopus.com/record/display.uri?eid=2-s2.0-7644240201&origin=inward&txGid=59a9d776a71da5b8565505988d27f04bhttps://repository.hanyang.ac.kr/handle/20.500.11754/151670
- ISSN
- 0378-0066
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML