다중검출용 바이오센싱 빛 스마트 약물전달을 위한 이방성 고분자 나노구조체 개발

Abstract

Anisotropic nano-architectures with different physicochemical properties have been recently developed as smart materials for biosensing, advanced drug delivery and tissue engineering. Fluorescence-based bicompartmental biohybrid nanoprobes for multiplexing detection of biological molecules were synthesized via the electrohydrodynamic (EHD) co-jetting of two polymer solutions with side-by-side needle geometry. The biocompatible polymer, poly(acrylamide-co-acrylic acid), poly(AAm-co-AA) was modified with maleimide- or acetylene group to introduce different targeting biological moieties into each compartment, respectively. Each compartments were crosslinked by thermal imidization and then stable in swollen state in physiologically relevant buffer. Two compartments were selectively bound to the respective antibody immobilized substrate in the presence of the antigens via the immunosandwich complex formation, confirmed by 3D sectioning of the confocal laser scanning microcopy (CLSM). These bicompartmental nanoprobes have great potential that the anisotropic particles having multiple targeting moieties and fluorescence dyes at each compartment could be very useful for efficient multiplexing sensing applications. In the other study thermally-responsive bicompartmental nanofiber scaffolds were produced through electrohydrodynamic (EHD) co-jetting with side by side needle geometry. One compartment with thermo-responsiveness is composed of poly(N-isopropylacrylamide-co-allylamine), Poly(NIPAM-co-AA) with acrylic moieties while the other compartment is made up of polyethylene glycol dimethacrylates, PEGDMA. Two distinct compartments were chemically-crosslinked by UV irradiation and then stable in the swollen state in physiologically relevant buffers. Each compartment loaded with different drugs could be released at different rates by diffusion and thermal actuation. These bicompartmental nanofiber scaffolds have great potential for multi-drug delivery reservoirs and tissue engineering scaffolds.