Cyclin-dependent kinase 저해 단백질 p16INK4A의 인체 암세포에서의 세포사멸 유도 활성
- Title
- Cyclin-dependent kinase 저해 단백질 p16INK4A의 인체 암세포에서의 세포사멸 유도 활성
- Other Titles
- A Cyclin-Dependent Kinase Inhibitor, pl6(INK4A), Induces Apoptosis in The Human Cancer Cells
- Author
- 이철훈
- Keywords
- P16(INK4A); Apoptosis; Cell cycle arrest
- Issue Date
- 2004-03
- Publisher
- 한국미생물생명공학회
- Citation
- 한국미생물·생명공학회지, v. 32, No. 1, Page. 72-77
- Abstract
- A Cyclin-Dependent Kinase Inhibitor, p16INK4A, Induces Apoptosis in The Human
Cancer Cells. Kim, Min-Kyoung and Chul-Hoon Lee*. Department of Medical Genetics,
Hanyang University College of Medicine, Seoul 133-791, Korea - Previously, we
synthesized a novel Cyclin-dependent kinase inhibitor, MCS-5A. Also, we investigated
the involvement of cell cycle regulatory events during MCS-5A-mediated apoptosis in
HL-60(+p16/-p53) cells with up-regulation of p16 protein expression. In contrast,
apoptosis was not observed in A549(-p16/+p53) cells. Therefore we propose that
p16INK4A is a key enzyme for inducing apoptosis. In the present studies, we have
explored the mechanism of p16INK4A-mediated cytotoxicity and the role of
p16INK4A overexpression in the induction of apoptosis in human tumor cells. The
tumor suppressor gene p16INK4A is known as a cyclin-dependent kinase inhibitor
(CKI) and cell cycle regulator. We expressed wild type p16INK4A in pcDNA3.1 vector
and then transfected into non-small cell lung cancer (NSCLC) cell expressing different
statue of p16INK4A, p53 gene[A549(-p16/+p53), H1299(-p16/-p53) and HeLa
(+p16/+p53)cell line]. TUNEL assay (including propidium iodide staining following
transfection of these cell line with pcDNA3.1-p16) indicate that p16INK4A-mediated
cytotoxicity was associated with apoptosis. This is supported by studies demonstrating
an induction of caspase 3 cleavage due to the transfection of A549, H1299 and HeLa
cells with pcDNA3.1-p16. These results suggest that p16INK4A has a new function of
inducing apoptosis which is not related with the function of tumor suppressor gene p.53
- URI
- http://kiss.kstudy.com/thesis/thesis-view.asp?key=2215861https://repository.hanyang.ac.kr/handle/20.500.11754/134677
- ISSN
- 1598-642x
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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