Optimization of hexanoic acid production in recombinant Escherichia coli by precise flux rebalancing
- Title
- Optimization of hexanoic acid production in recombinant Escherichia coli by precise flux rebalancing
- Author
- 상병인
- Keywords
- Hexanoic acid; Flux rebalancing; Acetyl-CoA acetyltransferase; 5 '-UTR; Acetyl-CoA transferase
- Issue Date
- 2018-07
- Publisher
- ELSEVIER SCI LTD
- Citation
- BIORESOURCE TECHNOLOGY, v. 247, page. 1253-1257
- Abstract
- The aim of this study is to demonstrate that rebalancing of metabolic fluxes at acetyl-CoA branch node can substantially improve the titer and productivity of hexanoic acid in recombinant Escherichia coli strains. First, a hexanoic acid-producing E. coli strain was constructed by expressing genes encoding beta-ketothiolase (BktB) from Cupriavidus necator and acetyl-CoA transferase (ACT) from Megasphaera sp. MH in a butyric acid producer strain. Next, metabolic flux was optimized at the acetyl-CoA branch node by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB). Four synthetic 5'- untranslated regions were designed for atoB using UTR Designer to modulate the expression level of the gene. Notably, the productivity of the optimized strain (14.7 mg/L/h) was the highest among recombinant E. coli strains in literature when using a similar inoculum size for fermentation. These results show that fine-tuning the expression level of atoB is critical for production of hexanoic acid.
- URI
- https://www.sciencedirect.com/science/article/pii/S096085241731831X?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/119483
- ISSN
- 0960-8524; 1873-2976
- DOI
- 10.1016/j.biortech.2017.10.014
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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