Photoautotrophic production of macular pigment in a Chlamydomonas reinhardtii strain generated by using DNA-free CRISPR-Cas9 RNP-mediated mutagenesis
- Title
- Photoautotrophic production of macular pigment in a Chlamydomonas reinhardtii strain generated by using DNA-free CRISPR-Cas9 RNP-mediated mutagenesis
- Author
- 배상수
- Keywords
- algal biotechnology; Chlamydomonas reinhardtii; CRISPR-Cas9; lutein; zeaxanthin
- Issue Date
- 2018-03
- Publisher
- WILEY
- Citation
- BIOTECHNOLOGY AND BIOENGINEERING, v. 115, no. 3, page. 719-728
- Abstract
- Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this study, we selected the C. reinhardtii CC-4349 strain as the best candidate among seven laboratory strains tested for carotenoid production. A knock-out mutant of the zeaxanthin epoxidase gene induced by preassembled DNA-free CRISPR-Cas9 ribonucleoproteins in the CC-4349 strain had a significantly higher zeaxanthin content (56-fold) and productivity (47-fold) than the wild type without the reduction in lutein level. Furthermore, we produced eggs fortified with lutein (2-fold) and zeaxanthin (2.2-fold) by feeding hens a diet containing the mutant. Our results clearly demonstrate the possibility of cost-effective commercial use of microalgal mutants induced by DNA-free CRISPR-Cas9 ribonucleoproteins in algal biotechnology for the production of high-value products.
- URI
- https://onlinelibrary.wiley.com/doi/abs/10.1002/bit.26499https://repository.hanyang.ac.kr/handle/20.500.11754/118098
- ISSN
- 0006-3592; 1097-0290
- DOI
- 10.1002/bit.26499
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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