Enhanced Delivery of Protein Fused to Cell Penetrating Peptides to Mammalian Cells
- Title
- Enhanced Delivery of Protein Fused to Cell Penetrating Peptides to Mammalian Cells
- Author
- 박장환
- Keywords
- Amodiaquine (AQ); Cell Penetrating Peptide (CPP); Differentiation; Polylysine (9K); Reprogramming
- Issue Date
- 2019-05
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Citation
- BMB REPORTS, v. 52, NO 5, Page. 324-329
- Abstract
- Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols.
- URI
- http://www.bmbreports.org/journal/view.html?doi=10.5483/BMBRep.2019.52.5.195https://repository.hanyang.ac.kr/handle/20.500.11754/111828
- ISSN
- 1976-6696; 1976-670X
- DOI
- 10.5483/BMBRep.2019.52.5.195
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
- Files in This Item:
- Enhanced Delivery of Protein Fused to Cell Penetrating Peptides to Mammalian Cells.pdfDownload
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