Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박장환 | - |
dc.date.accessioned | 2019-11-05T00:14:16Z | - |
dc.date.available | 2019-11-05T00:14:16Z | - |
dc.date.issued | 2019-05 | - |
dc.identifier.citation | BMB REPORTS, v. 52, NO 5, Page. 324-329 | en_US |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.issn | 1976-670X | - |
dc.identifier.uri | http://www.bmbreports.org/journal/view.html?doi=10.5483/BMBRep.2019.52.5.195 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/111828 | - |
dc.description.abstract | Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols. | en_US |
dc.description.sponsorship | This work was supported by St. Jude Institutional Funds (to M-J Han), the Global Excellent Technology Innovation Program (Project No. 10052590 to K. Han), the Bio & Medical Technology Development Program (NRF-2016M3A9B4918833 to C-H Park), and Basic Science Research Program (NRF-2016R1A2B4007640 to C-H Kim). | en_US |
dc.language.iso | en | en_US |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | en_US |
dc.subject | Amodiaquine (AQ) | en_US |
dc.subject | Cell Penetrating Peptide (CPP) | en_US |
dc.subject | Differentiation | en_US |
dc.subject | Polylysine (9K) | en_US |
dc.subject | Reprogramming | en_US |
dc.title | Enhanced Delivery of Protein Fused to Cell Penetrating Peptides to Mammalian Cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 5 | - |
dc.relation.volume | 52 | - |
dc.identifier.doi | 10.5483/BMBRep.2019.52.5.195 | - |
dc.relation.page | 324-329 | - |
dc.relation.journal | BMB REPORTS | - |
dc.contributor.googleauthor | Moon, Jung-Il | - |
dc.contributor.googleauthor | Han, Min-Joon | - |
dc.contributor.googleauthor | Yu, Shin-Hye | - |
dc.contributor.googleauthor | Lee, Eun-Hye | - |
dc.contributor.googleauthor | Kim, Sang-Mi | - |
dc.contributor.googleauthor | Han, Kyuboem | - |
dc.contributor.googleauthor | Park, Chang-Hwan | - |
dc.contributor.googleauthor | Kim, Chun-Hyung | - |
dc.relation.code | 2019037758 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | chshpark | - |
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