DNA의 제한효소 반응 및 전기영동 검출용 PDMS/유리 마이크로바이오칩

Abstract

This paper reports low-cost PDMS/glass based DNA microbiochip for the restriction enzyme reaction and its products detection using the capillary electrophoresis. The microbiochip (25㎜ × 75㎜) has the heater integrated reactor (5 ㎕) for DNA restriction enzyme reaction at 37 ℃ and the microchannel (80 ㎛ x100㎛ x58㎜) for the capillary electrophoresis detection. It is experimentally confirmed that the digestion of the plasmid (pGEM®-4Z) by the enzyme (Hind Ⅲ and Sca Ⅰ) is performed for less than 10 min and its electrophoresis detection is able to sequentially on the fabricated microbiochip.