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Nanopore Sensing in Aqueous Two-Phase System: Simultaneous Enhancement of Signal and Translocation Time via Conformal Coating

Title
Nanopore Sensing in Aqueous Two-Phase System: Simultaneous Enhancement of Signal and Translocation Time via Conformal Coating
Author
곽노균
Issue Date
2017-01
Publisher
WILEY-V C H VERLAG GMBH
Citation
SMALL, v. 13, no. 3, Article no. 1601725
Abstract
Nanofluidic resistive pulse sensing (RPS) has been extensively used to measure the size, concentration, and surface charge of nanoparticles in electrically conducting solutions. Although various methods have been explored for improving detection performances, intrinsic problems including the extremely low particle-to-pore volume ratio (<0.01%) and fast nanoparticle translocation (10-1000 mu s) still induce difficulties in detection, such as low signal magnitudes and short translocation times. Herein, we present an aqueous two-phase system (ATPS) in a nanofluidic RPS for amplifying translocation signals and decreasing translocation speeds simultaneously. Two immiscible aqueous liquids build a liquid-liquid interface inside nanopores. As particles translocate from a high-affinity liquid phase into a lower-affinity one, the high-affinity liquid forms a conformal coating on the particles, which increases the effective particle size and amplifies the current-blockage signal. The translocation time is also increased, as the ATPS interface impedes the particle translocation. For 20 nm particles, 7.92-fold and 5.82-fold enhancements of signal magnitude and translocation time can be achieved. To our knowledge, this is the first attempt to improve nanofluidic RPS by treating an interface of solution reservoirs for manipulating target particles rather than nanopores. This direct particle manipulation allows us to solve the two intrinsic problems all at once.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.201601725https://repository.hanyang.ac.kr/handle/20.500.11754/106103
ISSN
1613-6810; 1613-6829
DOI
10.1002/smll.201601725
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > MECHANICAL ENGINEERING(기계공학부) > Articles
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