Generation of Multilayered 3D Structures of HepG2 Cells Using a Bio-printing Technique
- Title
- Generation of Multilayered 3D Structures of HepG2 Cells Using a Bio-printing Technique
- Author
- 백승삼
- Keywords
- Hep G2 cell; Printing; three-dimensional
- Issue Date
- 2017-01
- Publisher
- EDITORIAL OFFICE GUT & LIVER
- Citation
- GUT AND LIVER, v. 11, no. 1, page. 121-128
- Abstract
- Background/Aims: Chronic liver disease is a major widespread cause of death, and whole liver transplantation is the only definitive treatment for patients with end-stage liver diseases. However, many problems, including donor shortage, surgical complications and cost, hinder their usage. Recently, tissue-engineering technology provided a potential breakthrough for solving these problems. Three-dimensional (3D) printing technology has been used to mimic tissues and organs suitable for transplantation, but applications for the liver have been rare. Methods: A 3D bioprinting system was used to construct 3D printed hepatic structures using alginate. HepG2 cells were cultured on these 3D structures for 3 weeks and examined by fluorescence microscopy, histology and immunohistochemistry. The expression of liver specific markers was quantified on days 1, 7, 14, and 21. Results: The cells grew well on the alginate scaffold, and liver-specific gene expression increased. The cells grew more extensively in 3D culture than two-dimensional culture and exhibited better structural aspects of the liver, indicating that the 3D bioprinting method recapitulates the liver architecture. Conclusions: The 3D bioprinting of hepatic structures appears feasible. This technology may become a major tool and provide a bridge between basic science and the clinical challenges for regenerative medicine of the liver.
- URI
- http://www.gutnliver.org/journal/view.html?doi=10.5009/gnl16010https://repository.hanyang.ac.kr/handle/20.500.11754/105156
- ISSN
- 1976-2283; 2005-1212
- DOI
- 10.5009/gnl16010
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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- Generation of Multilayered 3D Structures of HepG2 Cells Using a Bio-printing Technique.pdfDownload
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