Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 백승삼 | - |
dc.date.accessioned | 2019-05-21T06:00:41Z | - |
dc.date.available | 2019-05-21T06:00:41Z | - |
dc.date.issued | 2017-01 | - |
dc.identifier.citation | GUT AND LIVER, v. 11, no. 1, page. 121-128 | en_US |
dc.identifier.issn | 1976-2283 | - |
dc.identifier.issn | 2005-1212 | - |
dc.identifier.uri | http://www.gutnliver.org/journal/view.html?doi=10.5009/gnl16010 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/105156 | - |
dc.description.abstract | Background/Aims: Chronic liver disease is a major widespread cause of death, and whole liver transplantation is the only definitive treatment for patients with end-stage liver diseases. However, many problems, including donor shortage, surgical complications and cost, hinder their usage. Recently, tissue-engineering technology provided a potential breakthrough for solving these problems. Three-dimensional (3D) printing technology has been used to mimic tissues and organs suitable for transplantation, but applications for the liver have been rare. Methods: A 3D bioprinting system was used to construct 3D printed hepatic structures using alginate. HepG2 cells were cultured on these 3D structures for 3 weeks and examined by fluorescence microscopy, histology and immunohistochemistry. The expression of liver specific markers was quantified on days 1, 7, 14, and 21. Results: The cells grew well on the alginate scaffold, and liver-specific gene expression increased. The cells grew more extensively in 3D culture than two-dimensional culture and exhibited better structural aspects of the liver, indicating that the 3D bioprinting method recapitulates the liver architecture. Conclusions: The 3D bioprinting of hepatic structures appears feasible. This technology may become a major tool and provide a bridge between basic science and the clinical challenges for regenerative medicine of the liver. | en_US |
dc.description.sponsorship | This work was supported by the research fund of Hanyang University (HY-2014). | en_US |
dc.language.iso | en | en_US |
dc.publisher | EDITORIAL OFFICE GUT & LIVER | en_US |
dc.subject | Hep G2 cell | en_US |
dc.subject | Printing | en_US |
dc.subject | three-dimensional | en_US |
dc.title | Generation of Multilayered 3D Structures of HepG2 Cells Using a Bio-printing Technique | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 11 | - |
dc.identifier.doi | 10.5009/gnl16010 | - |
dc.relation.page | 121-128 | - |
dc.relation.journal | GUT AND LIVER | - |
dc.contributor.googleauthor | Jeon, Hyeryeon | - |
dc.contributor.googleauthor | Kang, Kyojin | - |
dc.contributor.googleauthor | Park, Su A. | - |
dc.contributor.googleauthor | Kim, Wan Doo | - |
dc.contributor.googleauthor | Paik, Seung Sam | - |
dc.contributor.googleauthor | Lee, Sang-Hun | - |
dc.contributor.googleauthor | Jeong, Jaemin | - |
dc.contributor.googleauthor | Choi, Dongho | - |
dc.relation.code | 2017005721 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | sspaik | - |
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