Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo
- Title
- Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo
- Author
- 조용우
- Keywords
- STEM-CELLS; STRATEGIES; THERAPY; PROLIFERATION; NANOPARTICLES; SELECTIVITY; CARTILAGE; SURVIVAL; DISEASE
- Issue Date
- 2016-03
- Publisher
- AMER CHEMICAL SOC
- Citation
- BIOCONJUGATE CHEMISTRY, v. 27, No. 4, Page. 927-936
- Abstract
- Establishment of an appropriate cell labeling and tracking method is essential for the development of cell-based therapeutic strategies. Here, we are introducing a new method for cell labeling and tracking by combining metabolic gylcoengineering and bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-D-mannosamine (Ac(4)ManNAz) to generate unnatural azide groups (-N-3) on the surface of the cells. Subsequently, the unnatural azide groups on the cell surface were specifically conjugated with near-infrared fluorescent (NIRF) dye-tagged dibenzyl cyclooctyne (DBCO-650) through bioorthogonal copper-free Click chemistry. Importantly, DBCO-650-labeled chondrocytes presented strong NIRF signals with relatively low cytotoxicity and the amounts of azide groups and DBCO-650 could be easily controlled by feeding different amounts of Ac4ManNAz and DBCO-650 to the cell culture system. For the in vivo cell tracking, DBCO-650-labeled chondrocytes (1 x 10(6) cells) seeded on the 3D scaffold were subcutaneously implanted into mice and the transplanted DBCO-650-labeled chondrocytes could be effectively tracked in the prolonged time period of 4 weeks using NIRF imaging technology. Furthermore, this new cell labeling and tracking technology had minimal effect on cartilage formation in vivo.
- URI
- https://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.6b00010https://repository.hanyang.ac.kr/handle/20.500.11754/102209
- ISSN
- 1043-1802
- DOI
- 10.1021/acs.bioconjchem.6b00010
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
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