Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 조용우 | - |
dc.date.accessioned | 2019-04-17T02:40:59Z | - |
dc.date.available | 2019-04-17T02:40:59Z | - |
dc.date.issued | 2016-03 | - |
dc.identifier.citation | BIOCONJUGATE CHEMISTRY, v. 27, No. 4, Page. 927-936 | en_US |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.uri | https://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.6b00010 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/102209 | - |
dc.description.abstract | Establishment of an appropriate cell labeling and tracking method is essential for the development of cell-based therapeutic strategies. Here, we are introducing a new method for cell labeling and tracking by combining metabolic gylcoengineering and bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-D-mannosamine (Ac(4)ManNAz) to generate unnatural azide groups (-N-3) on the surface of the cells. Subsequently, the unnatural azide groups on the cell surface were specifically conjugated with near-infrared fluorescent (NIRF) dye-tagged dibenzyl cyclooctyne (DBCO-650) through bioorthogonal copper-free Click chemistry. Importantly, DBCO-650-labeled chondrocytes presented strong NIRF signals with relatively low cytotoxicity and the amounts of azide groups and DBCO-650 could be easily controlled by feeding different amounts of Ac4ManNAz and DBCO-650 to the cell culture system. For the in vivo cell tracking, DBCO-650-labeled chondrocytes (1 x 10(6) cells) seeded on the 3D scaffold were subcutaneously implanted into mice and the transplanted DBCO-650-labeled chondrocytes could be effectively tracked in the prolonged time period of 4 weeks using NIRF imaging technology. Furthermore, this new cell labeling and tracking technology had minimal effect on cartilage formation in vivo. | en_US |
dc.description.sponsorship | This study was funded by Development of High Medical Technology Project (HI14C2755) of KHIDI, Global Research Laboratory Project (NRF-2013K1A1A2A02050115) of KNRF and the KIST Intramural project of Theranosis, Korea. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | STEM-CELLS | en_US |
dc.subject | STRATEGIES | en_US |
dc.subject | THERAPY | en_US |
dc.subject | PROLIFERATION | en_US |
dc.subject | NANOPARTICLES | en_US |
dc.subject | SELECTIVITY | en_US |
dc.subject | CARTILAGE | en_US |
dc.subject | SURVIVAL | en_US |
dc.subject | DISEASE | en_US |
dc.title | Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 27 | - |
dc.identifier.doi | 10.1021/acs.bioconjchem.6b00010 | - |
dc.relation.page | 927-936 | - |
dc.relation.journal | BIOCONJUGATE CHEMISTRY | - |
dc.contributor.googleauthor | Yoon, Hwa In | - |
dc.contributor.googleauthor | Yhee, Ji Young | - |
dc.contributor.googleauthor | Na, Jin Hee | - |
dc.contributor.googleauthor | Lee, Sangmin | - |
dc.contributor.googleauthor | Lee, Hyukjin | - |
dc.contributor.googleauthor | Kang, Sun-Woong | - |
dc.contributor.googleauthor | Chang, Hyeyoun | - |
dc.contributor.googleauthor | Ryu, Ju Hee | - |
dc.contributor.googleauthor | Lee, Seulki | - |
dc.contributor.googleauthor | Cho, Yong Woo | - |
dc.contributor.googleauthor | Kwon, Ick Chan | - |
dc.contributor.googleauthor | Kim, Kwangmeyung | - |
dc.relation.code | 2016000604 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF MATERIALS SCIENCE AND CHEMICAL ENGINEERING | - |
dc.identifier.pid | ywcho7 | - |
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