Lipid Extraction from Wet Biomass of Nannochloropsis sp Using Chloroform-Methanol Mixture
- Title
- Lipid Extraction from Wet Biomass of Nannochloropsis sp Using Chloroform-Methanol Mixture
- Author
- 강신춘
- Keywords
- Lipid Extraction; Nannochloropsis sp.; Microalgae; Chloroform-Methanol Mixture; RAPID METHOD; MICROALGAE; PURIFICATION; BIODIESEL
- Issue Date
- 2016-02
- Publisher
- SOC CHEMICAL ENG JAPAN
- Citation
- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, v. 49, No. 2, Page. 126-129
- Abstract
- Lipid extraction from microalgae using a chloroform-methanol mixture was performed to obtain optimum conditions for the isolation of total lipids. The effects of extraction parameters including proportions of methanol to chloroform and the ratios of total solvent volume to dry biomass on extraction yields were evaluated. In addition, the effect of water on extraction performance was investigated for the purpose of applications to extract lipids from wet microalgae. The optimum conditions maximizing lipid yields were as follows: the proportion of methanol to chloroform of 1:1 and the ratio of total solvent volume to dry biomass of 30 mL/g. Water positively affected lipid extraction efficiency when chloroform, methanol, and water formed a monophasic solution. Finally, the newly-developed method was compared with existing extraction methods.
- URI
- https://www.jstage.jst.go.jp/article/jcej/49/2/49_15we081/_article/-char/ja/https://repository.hanyang.ac.kr/handle/20.500.11754/102114
- ISSN
- 0021-9592
- DOI
- 10.1252/jcej.15we081
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
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