Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes
- Title
- Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes
- Author
- 백승삼
- Keywords
- Epigenetic reprogramming; Histone methylation; Nuclear transfer; Oocyte extract; Reprogrammed cells
- Issue Date
- 2012-11
- Publisher
- COMPANY OF BIOLOGISTS LTD, BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND
- Citation
- Development (Cambridge), Vol.139 No.23 [2012], 4330-4340
- Abstract
- Genomic reprogramming factors in the cytoplasm of germinal vesicle (GV) stage oocytes have been shown to improve the efficiency of producing cloned mouse offspring through the exposure of nuclei to a GV cytoplasmic extract prior to somatic cell nuclear transfer (SCNT) to enucleated oocytes. Here, we developed an extract of GV stage pig oocytes (GVcyto-extract) to investigate epigenetic reprogramming events in treated somatic cell nuclei. This extract induced differentiation-associated changes in fibroblasts, resulting in cells that exhibit pluripotent stem cell-like characteristics and that redifferentiate into three primary germ cell layers both in vivo and in vitro. The GVcyto-extract treatment induced large numbers of high-quality SCNT-generated blastocysts, with methylation and acetylation of H3-K9 and expression of Oct4 and Nanog at levels similar to in vitro fertilized embryos. Thus, GVcyto-extract could elicit differentiation plasticity in treated fibroblasts, and SCNT-mediated reprogramming reset the epigenetic state in treated cells more efficiently than in untreated cells. In summary, we provide evidence for the generation of stem-like cells from differentiated somatic cells by treatment with porcine GVcyto-extract.
- URI
- http://dev.biologists.org/content/139/23/4330.full
- ISSN
- 0950-1991; 1477-9129
- DOI
- 10.1242/dev.086116
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
- Files in This Item:
- 4330.full.pdfDownload
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