Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 백승삼 | - |
dc.date.accessioned | 2018-03-04T07:06:12Z | - |
dc.date.available | 2018-03-04T07:06:12Z | - |
dc.date.issued | 2012-11 | - |
dc.identifier.citation | Development (Cambridge), Vol.139 No.23 [2012], 4330-4340 | en_US |
dc.identifier.issn | 0950-1991 | - |
dc.identifier.issn | 1477-9129 | - |
dc.identifier.uri | http://dev.biologists.org/content/139/23/4330.full | - |
dc.description.abstract | Genomic reprogramming factors in the cytoplasm of germinal vesicle (GV) stage oocytes have been shown to improve the efficiency of producing cloned mouse offspring through the exposure of nuclei to a GV cytoplasmic extract prior to somatic cell nuclear transfer (SCNT) to enucleated oocytes. Here, we developed an extract of GV stage pig oocytes (GVcyto-extract) to investigate epigenetic reprogramming events in treated somatic cell nuclei. This extract induced differentiation-associated changes in fibroblasts, resulting in cells that exhibit pluripotent stem cell-like characteristics and that redifferentiate into three primary germ cell layers both in vivo and in vitro. The GVcyto-extract treatment induced large numbers of high-quality SCNT-generated blastocysts, with methylation and acetylation of H3-K9 and expression of Oct4 and Nanog at levels similar to in vitro fertilized embryos. Thus, GVcyto-extract could elicit differentiation plasticity in treated fibroblasts, and SCNT-mediated reprogramming reset the epigenetic state in treated cells more efficiently than in untreated cells. In summary, we provide evidence for the generation of stem-like cells from differentiated somatic cells by treatment with porcine GVcyto-extract. | en_US |
dc.language.iso | en | en_US |
dc.publisher | COMPANY OF BIOLOGISTS LTD, BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND | en_US |
dc.subject | Epigenetic reprogramming | en_US |
dc.subject | Histone methylation | en_US |
dc.subject | Nuclear transfer | en_US |
dc.subject | Oocyte extract | en_US |
dc.subject | Reprogrammed cells | en_US |
dc.title | Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes | en_US |
dc.type | Article | en_US |
dc.relation.no | 23 | - |
dc.relation.volume | 139 | - |
dc.identifier.doi | 10.1242/dev.086116 | - |
dc.relation.page | 4330-4340 | - |
dc.relation.journal | DEVELOPMENT | - |
dc.contributor.googleauthor | Kwon, Deug-Nam | - |
dc.contributor.googleauthor | Kang, Min-Hui | - |
dc.contributor.googleauthor | Oh, Mi-HyePark,Mi-RyungPark,Woo-JinNguyen Van ThuanKim,Jin-HoiPaik,Seung-Sam | - |
dc.relation.code | 2012202530 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | sspaik | - |
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