PLD1 activation mediates Amb a 1-induced Th2-associated cytokine expression via the JNK/ATF-2 pathway in BEAS-2B cells

Title
PLD1 activation mediates Amb a 1-induced Th2-associated cytokine expression via the JNK/ATF-2 pathway in BEAS-2B cells
Author
오재원
Keywords
Ambrosia artemisiifolia; PLD1; JNK; p70S6K; IL-13; IL-5
Issue Date
2015-08
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
CELLULAR IMMUNOLOGY, v. 298, NO 1-2, Page. 9-17
Abstract
The purpose of this study was to identify the role of phospholipase D1 (PLD1) in Amb a 1-induced IL-5 and IL-13 expression. When BEAS-2B cells were stimulated with Amb a 1, PLD activity increased, and knockdown of PLD1 decreased Amb a 1-induced IL-5 and IL-13 expression. Amb a 1 also activated the PLC gamma/p70S6K/JNK pathway. Furthermore, Amb a 1-induced PLD activation was also attenuated by PLC gamma inhibition, and knockdown of PLD1 decreased Amb a 1-induced activation of P70S6K and JNK. When ATF-2 activity was blocked with ATF-2 siRNA, Amb a 1-induced IL-5 and IL-13 expression was completely abolished, indicating that ATF-2 is a transcriptional factor required for the expression of IL-5 and IL-13 in response to Amb a 1. Taken together, we suggest that PLD1 acts as an important regulator in Amb a 1-induced expression of IL-5 and IL-13 via a PLC gamma/p70S6K/JNK/ATF-2 pathway in BEAS-2B cells. (C) 2015 Elsevier Inc. All rights reserved.
URI
http://www.sciencedirect.com/science/article/pii/S0008874915300095http://hdl.handle.net/20.500.11754/26804
ISSN
0008-8749; 1090-2163
DOI
10.1016/j.cellimm.2015.08.003
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE