Effect-directed identification of novel aryl hydrocarbon receptor-active aromatic compounds in coastal sediments collected from a highly industrialized area
- Title
- Effect-directed identification of novel aryl hydrocarbon receptor-active aromatic compounds in coastal sediments collected from a highly industrialized area
- Author
- 문효방
- Keywords
- Aryl hydrocarbon receptor; H4IIE-luc bioassay; GC-QTOFMS; Full-scan screening analysis; Industrial area
- Issue Date
- 2022-01
- Publisher
- ELSEVIER
- Citation
- SCIENCE OF THE TOTAL ENVIRONMENT, v. 803, article no. 149969, Page. 1.0-9.0
- Abstract
- In this study, we identified major aryl hydrocarbon receptor (AhR) agonists in the sediments from Yeongil Bay (n = 6) using effect-directed analysis. Using the H4IIE-luc bioassays, great AhR-mediated potencies were found in aro-matic fractions (F2) of sediment organic extracts from silica gel column chromatography and sub-fractions (F2.6- F2.8) from reverse phase-HPLC. Full-scan mass spectrometric analysis using GC-QTOFMS was conducted to identify novel AhR agonists in highly potent fractions, such as F2.6-F2.8 of S1 (Gumu Creek). Selection criteria for AhR-active compounds consisted of three steps, including matching factor of NIST library (>_70), aromatic structures, and the number of aromatic rings (>_4). Fifty-nine compounds were selected as tentative AhR agonist candidates, with the AhR-mediated activity being assessed for six compounds for which standard materials were available commercially. Of these compounds, 20-methylcholanthrene, 7-methylbenz[a]anthracene, 10-methylbenz[a]pyrene, and 7,12-dimethylbenz[a]anthracene exhibited significant AhR-mediated potency. Relative potency values of these com-pounds were determined relative to benzo[a]pyrene to be 3.2, 1.4, 1.2, and 0.2, respectively. EPA positive matrix fac-torization modeling indicated that the sedimentary AhR-active aromatic compounds primarily originated from coal combustion and vehicle emissions. Potency balance analysis indicated that four novel AhR agonists explained 0.007% to 1.7% of bioassay-derived AhR-mediated potencies in samples. (c) 2021 Elsevier B.V. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S0048969721050440?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/182251
- ISSN
- 0048-9697;1879-1026
- DOI
- 10.1016/j.scitotenv.2021.149969
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MARINE SCIENCE AND CONVERGENCE ENGINEERING(해양융합공학과) > Articles
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