Berberine Ameliorates Brain Inflammation in Poloxamer 407-Induced Hyperlipidemic Rats
- Title
- Berberine Ameliorates Brain Inflammation in Poloxamer 407-Induced Hyperlipidemic Rats
- Author
- 홍민하
- Keywords
- Hyperlipidemia; Berberine; Poloxamer; Apoptosis; Glial fibrillary acidic protein; Ibal
- Issue Date
- 2019-11
- Publisher
- KOREAN CONTINENCE SOC
- Citation
- INTERNATIONAL NEUROUROLOGY JOURNAL, v. 23, page. 102-110
- Abstract
- Purpose: Hyperlipidemia, which promotes the development of atherosclerosis, ischemic stroke, and other forms of brain injury, can be induced by poloxamer-407. Berberine is a primary pharmacological active component of Coptidis Rhizoma that has a number of therapeutic activities. This study investigated the effects of berberine on poloxamer-407-induced brain inflammation by evaluating its effects on short-term memory, cell proliferation, inflammation, and apoptosis in the hippocampus.
Methods: To induce hyperlipidemia in a rat model, 500 mg/kg of poloxamer-407 was injected intraperitoneally. Berberine was orally administered to the rats in the berberine-treated groups once a day for 4 weeks. The step-down task avoidance task was performed to measure short-term memory. An analysis of serum lipids, immunohistochemistry for 5-bromo-2'-deoxyuridine, glial fibrillary acidic protein (GAP), and ionized calcium-binding adapter molecule 1 (Ibal) in the dentate gyrus, and western blot analysis for Bax, Bcl-2, and cytochrome c in the hippocampus were performed.
Results: In hyperlipidemic rats, berberine reduced the levels of triglycerides, total cholesterol, and low-density lipoprotein cholesterol and increased the level of high-density lipoprotein cholesterol in hyperlipidemic rats. Berberine also increased cell proliferation and short-term memory, as well as decreasing the expression of GFAP, Ibal, Bax, and cytochrorne c and increasing Bcl-2 expression.
Conclusions: Berberine treatment improved short-term memory in hyperlipidemia by increasing neuronal proliferation and inhibiting neuronal apoptosis. Berberine treatment also improved lipid metabolism.
- URI
- https://www.einj.org/journal/view.php?doi=10.5213/inj.1938216.108https://repository.hanyang.ac.kr/handle/20.500.11754/155649
- ISSN
- 2093-4777; 2093-6931
- DOI
- 10.5213/inj.1938216.108
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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