Cryopreserved cord blood mononuclear cells in DMSO are healthy for at least 6 hours after thawing
- Title
- Cryopreserved cord blood mononuclear cells in DMSO are healthy for at least 6 hours after thawing
- Author
- 김용주
- Keywords
- Cord blood; Cryopreservation; Dimethyl sulfoxide; Viability; Apoptosis
- Issue Date
- 2020-02
- Publisher
- Elsevier Ltd.
- Citation
- Transfusion and Apheresis Science, v. 59, no. 1, article no. UNSP 102603
- Abstract
- Purposes: We investigated the impact of time, storage temperature, and dimethyl sulfoxide (DMSO) on the viability of HSCs, as well as on apoptotic changes in thawed CB.
Materials & Methods: Thirteen units of cryopreserved CB were thawed and half of each sample was stored at room temperature (RT) and the other half at 4 degrees C, without removing or diluting DMSO. Flow cytometry was employed to enumerate total nucleated cells (TNCs), total/viable CD34+ cells, and early/late apoptotic cells using anti-CD45, anti-CD34, and annexin V(AnV), 7-amino actinomycin D(AAD) staining, respectively.
Results: In CBs stored at 4 degrees C there were no significant changes in numbers of TNCs, total/viable CD34(+) cells, or early/late apoptotic cell up to 48 h. However, the numbers of these cells declined significantly at RT. Total and viable CD34(+) cell counts did not change for up to 6 h at RT but viable CD34+ cells decreased significantly after 24 h, and total CD34+ cell after 48 h. Early and late apoptosis tended to increase with time at RT, and numbers of viable CD34+ cells and early apoptotic cells differed significantly between RT and 4 degrees C after 48 h.
Conclusions: There are no significant changes of viability and apoptosis in CBs stored in DMSO at 4 degrees C until 48 h after thawing, while at RT, there are no significant changes of total/viable CD34+ cell counts or in the proportion of apoptotic cells for at least 6 h after thawing.
- URI
- https://www.sciencedirect.com/science/article/pii/S147305021930134X?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/152217
- ISSN
- 1473-0502
- DOI
- 10.1016/j.transci.2019.06.028
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML