Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김용주 | - |
dc.date.accessioned | 2020-08-12T07:51:45Z | - |
dc.date.available | 2020-08-12T07:51:45Z | - |
dc.date.issued | 2020-02 | - |
dc.identifier.citation | Transfusion and Apheresis Science, v. 59, no. 1, article no. UNSP 102603 | en_US |
dc.identifier.issn | 1473-0502 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S147305021930134X?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/152217 | - |
dc.description.abstract | Purposes: We investigated the impact of time, storage temperature, and dimethyl sulfoxide (DMSO) on the viability of HSCs, as well as on apoptotic changes in thawed CB. Materials & Methods: Thirteen units of cryopreserved CB were thawed and half of each sample was stored at room temperature (RT) and the other half at 4 degrees C, without removing or diluting DMSO. Flow cytometry was employed to enumerate total nucleated cells (TNCs), total/viable CD34+ cells, and early/late apoptotic cells using anti-CD45, anti-CD34, and annexin V(AnV), 7-amino actinomycin D(AAD) staining, respectively. Results: In CBs stored at 4 degrees C there were no significant changes in numbers of TNCs, total/viable CD34(+) cells, or early/late apoptotic cell up to 48 h. However, the numbers of these cells declined significantly at RT. Total and viable CD34(+) cell counts did not change for up to 6 h at RT but viable CD34+ cells decreased significantly after 24 h, and total CD34+ cell after 48 h. Early and late apoptosis tended to increase with time at RT, and numbers of viable CD34+ cells and early apoptotic cells differed significantly between RT and 4 degrees C after 48 h. Conclusions: There are no significant changes of viability and apoptosis in CBs stored in DMSO at 4 degrees C until 48 h after thawing, while at RT, there are no significant changes of total/viable CD34+ cell counts or in the proportion of apoptotic cells for at least 6 h after thawing. | en_US |
dc.description.sponsorship | This work was supported by the Research Fund of Hanyang University (HY-2016) | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Ltd. | en_US |
dc.subject | Cord blood | en_US |
dc.subject | Cryopreservation | en_US |
dc.subject | Dimethyl sulfoxide | en_US |
dc.subject | Viability | en_US |
dc.subject | Apoptosis | en_US |
dc.title | Cryopreserved cord blood mononuclear cells in DMSO are healthy for at least 6 hours after thawing | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 58 | - |
dc.identifier.doi | 10.1016/j.transci.2019.06.028 | - |
dc.relation.page | 1-4 | - |
dc.relation.journal | Transfusion and Apheresis Science | - |
dc.contributor.googleauthor | Lee, Young-Ho | - |
dc.contributor.googleauthor | Koh, Hani | - |
dc.contributor.googleauthor | Nam, Eunwoo | - |
dc.contributor.googleauthor | Kim, Yong Joo | - |
dc.relation.code | 2019032438 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | kyjoo | - |
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