Fast Cyclic Square-Wave Voltammetry To Enhance Neurotransmitter Selectivity and Sensitivity
- Title
- Fast Cyclic Square-Wave Voltammetry To Enhance Neurotransmitter Selectivity and Sensitivity
- Author
- 정승준
- Keywords
- CARBON-FIBER MICROELECTRODES; PAIRED-PULSE VOLTAMMETRY; REAL-TIME MEASUREMENT; EXTRACELLULAR DOPAMINE; BRAIN; ADSORPTION; FORM; QUANTIFICATION; FLUCTUATIONS; ADENOSINE
- Issue Date
- 2018-11
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v. 90, no. 22, page. 13348-13355
- Abstract
- Although fast-scan cyclic voltammetry (FSCV) has been widely used for in vivo neurochemical detection, the sensitivity and selectivity of the technique can be further improved. In this study, we develop fast cyclic square-wave voltammetry (FCSWV) as a novel voltammetric technique that combines large-amplitude cyclic square-wave voltammetry (CSWV) with background subtraction. A large-amplitude, square-shaped potential was applied to induce cycling through multiple redox reactions within a square pulse to increase sensitivity and selectivity when combined with a two-dimensional voltammogram. As a result, FCSWV was significantly more sensitive than FSCV (n = 5 electrodes, two-way ANOVA, p = 0.0002). In addition, FCSWV could differentiate dopamine from other catecholamines (e.g., epinephrine and norepinephrine) and serotonin better than conventional FSCV. With the confirmation that FCSWV did not influence local neuronal activity, despite the large amplitude of the square waveform, it could monitor electrically induced phasic changes in dopamine release in rat striatum before and after injecting nomifensine, a dopamine reuptake inhibitor.
- URI
- https://pubs.acs.org/doi/10.1021/acs.analchem.8b02920https://repository.hanyang.ac.kr/handle/20.500.11754/120545
- ISSN
- 0003-2700; 1520-6882
- DOI
- 10.1021/acs.analchem.8b02920
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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