Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 류종석 | - |
dc.date.accessioned | 2018-10-10T23:56:37Z | - |
dc.date.available | 2018-10-10T23:56:37Z | - |
dc.date.issued | 2009-10 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v. 388, No. 2, Page. 306-310 | en_US |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0006291X09015526?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/76420 | - |
dc.description.abstract | Protein misfolding cyclic amplification (PMCA) is a cell-free assay mimicking the prion replication process. However, constraints affecting PMCA have not been well-defined. Although cellular prion protein (PrP(C)) is required for prion replication, the influence of PrP(C) abundance on PMCA has not been assessed. Here, we show that PMCA was enhanced by using mouse brain material in which PrP(C) was overexpressed. Tg(MoPrP)4112 mice overexpressing PrP(C) supported more sensitive and efficient PMCA than wild type mice. As brain homogenate of Tg(MoPrP)4112 mice was diluted with PrP(C)-deficient brain material, PMCA became less robust. Our studies suggest that abundance of PrP(C) is a determinant that directs enhancement of PMCA. PMCA established here will contribute to optimizing conditions to enhance PrP(Sc) amplification by using concentrated PrP(C) source and expands the use of this methodology. (C) 2009 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | The authors thank Dr. Stanley Prusiner for providing the cosSHa.Tet cosmid expression vector and Prnpo/o mice to GT and Ms. Paula Thomason for editing this manuscript. This work was partially supported by the funds from the Sanders Brown Center on Aging and College of Medicine, University of Kentucky (CR), and from the NIH Grants 2RO1 NS040334-04 and 1P01AI077774-015261 (GT). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
dc.subject | Prion | en_US |
dc.subject | Transmissible spongiform encephalopathy | en_US |
dc.subject | PrP(C) | en_US |
dc.subject | PrP(Sc) | en_US |
dc.subject | Protein misfolding cyclic amplification | en_US |
dc.subject | Transgenic mice | en_US |
dc.title | Enhancement of protein misfolding cyclic amplification by using concentrated cellular prion protein source | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 388 | - |
dc.identifier.doi | 10.1016/j.bbrc.2009.07.163 | - |
dc.relation.page | 306-310 | - |
dc.relation.journal | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.contributor.googleauthor | Mays, Charles E. | - |
dc.contributor.googleauthor | Titlow, William | - |
dc.contributor.googleauthor | Seward, Tanya | - |
dc.contributor.googleauthor | Telling, Glenn C. | - |
dc.contributor.googleauthor | Ryou, Chongsuk | - |
dc.relation.code | 2009201235 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | cryou2 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.